Figure 3.

KM12, H460 and H810 cells were plated in 6-well plates at a density of 1.0×10⁶ cells/well at 24 h after being treated with AZD7451 at variable doses (1, 2.5, 4, 5, 7.5 and 10 nM). DMSO was used as a negative control. After 24 h, the cells were counted using a hemocytometer. (A) KM12 cell proliferation was potently inhibited at a concentration of 1 nM. Further cell proliferation was inhibited up to 2 nM, corresponding to a growth suppression of ~60%. (B) H460 cell proliferation was also inhibited at concentrations ≥1 nM. Further cell proliferation was inhibited up to 5 nM, also with a maximal suppression of growth of ~60%. (C) There was no apparent inhibitory effect on H810 cell proliferation. %control, ratio of cells treated with AZD7451 against control cells.