Figure 4.

In order to determine TRKA/B phosphorylation and the downstream signaling effects on Akt and ERK, western blotting was performed in KM12, H460 and H810 cells. KM12 and H460 cells were treated with AZD7451 at concentrations of 0, 1 and 5 nM, and incubated for 24 h. H810 cells were also used as control, after being treated at concentrations of 0 and 5 nM (C). In KM12 cells, the potent inhibition of pTRKA Tyr490 and pAkt was found to be concentration-dependent; however, the inhibition of the expression of pTRKA Tyr674/675 and pERK was unclear. In H460 cells, total TRKB was strongly expressed compared to total TRKA. In addition, the expression of pTRKB Tyr706/707 and pERK was inhibited in a drug concentration-dependent manner, but inhibition of the expression of pTRKB Tyr512 and pAkt was unclear in H460 cells. H810 cells exhibited little to no expression of TRKA/B.