Fig. 2.

αB-Crystallin inhibits cell growth and cell cycle progression in MEFs immortalized by p53 inactivation. a Cell number of immortalized WT and αB-crystallin ^−/− KO MEFs stably expressing DN p53 (abbreviated WT DN p53 and KO DN p53 MEFs, respectively) grown in subconfluent culture with the indicated passage (p) number (p1 at initial plating) reflecting 3-day growth periods (mean ± SD, n = 3). b Immortalized WT and αB-crystallin^−/− KO DN p53 MEFs were cultured in media containing 10 or 0.1 % FCS, labeled with BrdU, and the percentage of BrdU positive MEFs was determined by immunohistochemistry (mean ± SD, n = 3). c Flow cytometry analysis of DNA content in immortalized WT and αB-crystallin^−/− KO DN p53 MEFs cultured in media containing 0.1 % FCS. d Western blot analysis of paired primary WT and αB-crystallin^−/− KO MEFs grown in media containing 10 % FCS for 72 h or immortalized WT and αB-crystallin^−/− KO DN p53 MEFs grown in media containing 10 % FCS or 0.1 % FCS for 72 h. In a–c, *P < 0.05, **P < 0.01, or ***P < 0.001 versus control WT DN p53 MEFs