Fig. 4.

αB-Crystallin inhibits E1A-induced caspase-3 activation in response to chemotherapy drugs. a Immunoblot analysis of whole cell lysates from immortalized WT and αB-crystallin^−/− KO DN p53 MEFs (Control) or immortalized WT and αB-crystallin^−/− KO DN p53 MEFs stably expressing E1A. b and c WT E1A DN p53 MEFs and αB-crystallin^−/− KO E1A DN p53 MEFs were treated with 125 nM Taxol (b) or 250 nM Doxorubicin (c) for 12 h, and caspase-3 activity was measured using a colorimetric assay and normalized to levels present in WT E1A DN p53 MEFs (mean ± SD, n = 3).

*P < 0.05 versus control WT E1A DN p53 MEFs