Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Circ Cardiovasc Genet. 2014 May 13;7(3):257–265. doi: 10.1161/CIRCGENETICS.113.000455

Figure 2.

Figure 2

Defects in Diaphragm Development in B+/BR709C and BR709C/BR709C embryos. a-i. Immunofluorescence confocal images of E13.5 mouse sagittal sections near the middle of the torso stained for NMHCII-A (green) and striated muscle myosin (MF20, red) show loss of skeletal muscle cells in the lateral-most region of the diaphragm in B+/BR709C and BR709C/BR709C embryos (b,c, yellow boxes; enlarged in e,f). In the B+/B+ embryo skeletal muscle cells are numerous in this region (a, yellow box; enlarged in d). Skeletal muscle cells accumulated near the midline of the B+/BR709C and BR709C/BR709C diaphragm (b,c, white boxes; enlarged in h,i) compared to the B+/B+ diaphragm (a, white box; enlarged in g). DAPI (blue) stains nuclei. Scale bars: a-c, 200 μm; d-i, 50 μm.