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. 2014 Jul 22;9(7):e101959. doi: 10.1371/journal.pone.0101959

Figure 1. Effect of AN components on cytotoxicity, COX-2 expression and prostanoids production of GK and its modulation by catalase and PBL extract.

Figure 1

(A) Stimulation of PGF2α production of GK by ANE (50–1200 µg/ml) (n = 9). (B) Effect of arecoline on PGF2α production of GK (n = 3), (C) The regulation of ANE-induced PGE2 production of GK by catalase (500 and 1000 U/ml) (n = 11), (D) Catalase inhibited the ANE-induced COX-2 protein expression in GK as analyzed by western blotting. One representative western blotting picture was shown. (E) Catalase attenuated the ANE-induced cytotoxicity to GK (n = 7). (F) Effect of catechol on PGE2 production of GK (n = 4), (G) Effect of PBL extract on PGE2 production of GK (n = 3). Results were expressed as concentration (pg/ml) in the culture medium. (H) Cytotoxicity of ANE to GK (n = 6), (I) Cytotoxicity of arecoline to GK (n = 4), (J) Cytotoxicity of catechol to GK (n = 3). Results were expressed as MTT reduction (% of control, Mean ± SE). *denotes significant difference when compared with control. #denotes statistical significant difference when compared with ANE (800 µg/ml) solely-treated group (P<0.05).