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. 2014 Jul 15;127(14):3039–3051. doi: 10.1242/jcs.140434

Fig. 3.

Fig. 3.

RhoJ regulates focal adhesion size. (A) HUVECs were transfected with control siRNA (siControl) or RhoJ siRNA duplexes and after 48 hours were stained with vinculin-specific antibodies. Similarly, HUVECs transduced to express GFP or GFP–daRhoJ were fixed and stained for vinculin. The box indicates the enlarged area. Scale bar: 20 µm. (B) Focal adhesion areas from GFP- and GFP–daRhoJ-expressing HUVECs were measured using ImageJ of 15 focal adhesions per cell from a total of three cells, and the mean focal adhesion area calculated for each condition. This was performed three times and plotted are the mean values from each experiment (n = 3) (mean±s.e.m.). *P<0.05 (Student's t-test). (C) Similarly, focal adhesion areas from HUVECs transfected with siControl or RhoJ siRNA were measured using ImageJ of 106–135 focal adhesions from a total of five or six cells and mean focal adhesion area calculated per condition, plotted are the mean focal adhesion areas from three independent experiments (n = 3) (mean±s.e.m.). Reductions in focal adhesion areas were observed in each of the three experiments.