Fig. 4.

GFP–daRhoJ expression increases recruitment of partner proteins to focal adhesions. (A) HUVECs were transduced to express GFP or GFP–daRhoJ, and were fixed and stained for vinculin and either β-PIX, GIT1 or GIT2. Scale bar: 20 µm. (B) Cellular lysates were prepared from HUVECs expressing GFP or GFP–daRhoJ and western blotted for GIT2 phosphorylated on Y392 (pGIT2), GIT2, GFP and tubulin. (C) HUVECs were transfected with control siRNA (siControl) or RhoJ siRNA duplexes. After 48 hours, cells were lysed and blotted for GIT2 phosphorylated on Y392, GIT2, RhoJ and tubulin. These data are representative of three independent experiments.