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. 2014 Jul 22;9(7):e101602. doi: 10.1371/journal.pone.0101602

Figure 4. A. The Suppression of VEGF-Induced NFκB Transcriptional Activity by YC-1 in rho/VEGF Mouse Model.

Figure 4

The graph illustrates the suppression of NFκB DNA-binding activity by YC-1 in the retinas from different subgroups of rho/VEGF transgenic mice as compared to normal C57BL/6 mice. ELISA assay was done after 18 hours of incubation with YC-1, or SN50, or DMSO, or SN50M. Columns represent the means derived from three individual experiments. *P<0.05; **P<0.01; ***P<0.001, as compared with controls (C57BL/6 mice). The bars in show the mean (±S.E.M.) of NFκB transcriptional activity, which was significantly less in eyes treated with YC-1 and SN50 as compared to the eyes that were treated with DMSO or SN50M. B. The Role of VEGF on HIF-1α Transcriptional Activity in rho/VEGF Mouse Model. The DNA binding activity of HIF-1α was evaluated using an HIF-1α transcription factor assay kit. Our results demonstrated that VEGF overexpression in the rho/VEGF mouse didn't induce HIF-1α transcriptional activity, whereas this activity was inhibited by the use of YC-1.