Figure 3. Minor effect of Treg depletion on M. bovis BCG bacterial burden.

(A) DEREG mice were infected i.v. with 2×10⁶ CFU M. bovis BCG and Tregs were depleted by DT administration on days 2/3, 9/10 or 14/15 (black dots) in a single-depletion round or left untreated (white dots). CFU were determined in lungs, spleen and liver 20 days p.i.. Each symbol represents an individual mouse. Data represent mean ± SD of 3 mice per group. N = 1. (B) DEREG mice were infected i.v. with 2×10⁶ CFU M. bovis BCG and Tregs were depleted by DT administration on days 7/8 and 14/15 (black dots) in two rounds of depletion or were left untreated (white dots). CFU were assessed in lungs, spleen and liver on day 20 p.i.. Each symbol represents an individual mouse. Data represent mean ± SD of 3–5 mice per group. N = 3.