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. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084

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© 2014 Fujita, Fujii

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression of 3xFLAG-dCas9 in HT1080-derived cells. Expression of 3xFLAG-dCas9 was detected by immunoblot analysis with anti-FLAG Ab. Coomassie Brilliant Blue (CBB) staining is shown as a protein loading control. (B) Upper panel: Yield of enChIP analysis for the target site and potential off-target sites (mean +/- SD, n = 3). Lower panel: Alignment of the target site and potential off-target sites. The PAM sequences and mismatches are shown in blue and red, respectively.