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. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084

Figure 3. IFNγ-induced increased association of candidate proteins with the IRF-1 promoter.

Figure 3

(A) A scheme of human IRF-1 promoter. TSS: transcription start site; gRNA target: the position of the target sequence of gRNA. The positions of PCR primers for ChIP with distances from TSS are indicated. The results of ChIP using (B) anti-RBBP4 Ab, (C) anti-PA2G4 Ab, and (D) negative control normal IgG, are shown (mean +/- SD, n = 3). IFNγ-induced increase in binding of RBBP4 and PA2G4 to the IRF-1 promoter was reproducibly detected by ChIP analysis.