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. 2014 Jul 22;9(7):e103033. doi: 10.1371/journal.pone.0103033

Figure 3. FTY720 results in caspase-dependent cell apoptosis.

Figure 3

Western blotting assay was used to analyze the activation of caspases in Kasumi-1 cells treated with FTY720. Kasumi-1 cells were treated with indicated concentrations of FTY720 for 24 h. Caspase-3 (casp-3) and activated caspase-3 (c-casp-3) were shown by the same antibody blotting (A), caspase-8 (casp-8), active caspase-8 (c-casp-8), caspase-9 (casp-9) and active caspase-9 (c-casp-9) were analyzed by indicated antibodies blotting (B). (C) Kasumi-1 cells were pretreated with DEVD or VAD for 3 h, and subjected to 7.5 µM FTY720 for another 21 h. The effects of DEVD and VAD on FTY720-induced apoptosis were evaluated by flow cytometry. *P<0.05. (D) Kasumi-1 cells were treated with 7.5 µM FTY720 for indicated times, and then western blotting assay was carried out to analysis casp-3, casp-8, and casp-9 activation in Kasumi-1 cells treated with FTY720 for different time.