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. 2014 Jul 22;9(7):e103104. doi: 10.1371/journal.pone.0103104

Figure 2. Effect of the rs5029924 polymorphism on in vitro LPS stimulation of whole blood.

Figure 2

a) After LPS stimulation, mRNA and protein expression levels of TNFAIP3 were significantly different between individuals harboring the CC and individuals harboring the CT+TT genotypes (p = 0.012 and 0.007, respectively). b) NF-κB bound to specific DNA sequences were determined by electrophoretic mobility shift assays on polyacrylamide gels, after LPS stimulation. The IOD of NF-κB complex in individuals harboring the TT was significantly higher than the CC and TT genotype (p<0.001), and no significantly difference was recorded between the CC and TT genotype (p = 0.065). c) The TNF-α and IL-1β levels in supernatants were significantly different between individuals harboring the CC or the CT+TT genotypes after LPS stimulation (p = 0.001 and p = 0.011, respectively). * p<0.05, ** p<0.01 compared with the CC genotype.