Fig 2.
The putative DHCR24 promoter is well conserved in the chicken (Gg), mouse (Mm) and human (Hs) genomes. The sequences in bold type represent the three REST/NSRF binding sites in the mouse (RE1a, RE1b and RE1c) and the one in the human (RE1b) gene predicted by MatInspector. F and R and the dashed lines indicate the sequences of the respective forward and reverse primers used to amplify the region bracketing the three suspected REST binding domain(s) for the ChIP assay. The vertical arrowhead marks the likely translation start site. In particular, the RE1b sequence, although not suggested by MatInspector as a REST/NSRF binding sequence in the chicken, is remarkably similar to both mouse and human.