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. 2014 Jul 22;9(7):e103187. doi: 10.1371/journal.pone.0103187

Figure 3. iCLU localizes to the cytoskeleton fraction and is a Tau-interacting protein.

Figure 3

(a) Subcellular fractionation of HEK 293T after transfection with full-length CLU cDNA indicated that most of iCLU was found in the cytoskeleton/insoluble fraction. (b) Transfection of sCLU or iCLU cDNA in Tau-inducible HEK293 cells (iTau-HEK) followed by pull-down with anti-Tau antibody (HT7) showed co-immunoprecipitation of iCLU. (c) Transient co-transfection of Flag-tagged iCLU construct with WT or P301L mutant full-length Tau in HEK 293T cells followed by pull-down with anti-Tau antibody (HT7) or anti-Flag antibody showed reciprocal co-immunoprecipitation of iCLU and Tau, respectively. Conversely, transient co-transfection of Flag-tagged iCLU with WT or P301L 4-repeat microtubule binding domain (MBD) of Tau alone did not result in co-immunoprecipitation of Tau by anti-Flag antibody.