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. 2014 Jul 22;9(7):e103187. doi: 10.1371/journal.pone.0103187

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© 2014 Zhou et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Co-expression of each of the ten BIN1 human isoforms with iCLU in iTau-HEK cells revealed that iCLU interacted only with brain-specific BIN1 isoforms 1–3 containing a putative coiled-coil motif within the BAR domain (black arrows); however, all isoforms of BIN1 showed interaction with Tau (white arrows). (b) Western blotting of lysates immunoprecipitated with anti-Tau HT7 antibody indicated that both iCLU and BIN1.1 were co-immunoprecipitated from both PBS and dox-treated cells. (c) Western blotting from BIN1 99 antibody pull-down showed co-precipitation of iCLU in both PBS and dox conditions, but Tau bands were only detected in dox-treated cells. (d) Western blotting of anti-CLU C18 antibody immunoprecipitates showed similar bands for BIN1.1 in PBS and dox-treated cells.