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. 2014 Jul 22;9(7):e103187. doi: 10.1371/journal.pone.0103187

Figure 5. Expression and subcellular localization of CLU and BIN1 isoforms in the human cortex.

Figure 5

(a) Western blotting detection of CLU and BIN1 isoforms found in human brain lysates. (b) Western blotting analysis of human neuronal and astrocyte primary cultures revealed significant detection of iCLU forms in both cell types. (c) Cultured human neurons express primarily brain-specific BIN1 isoform 1, and appear to lack the shorter ubiquitous BIN1 isoforms 9 and 10, which are primarily represented in astrocytes. (d) Subcellular fractionation of human brain lysates showing that BIN1.1 and CLU co-localize with Tau and p-Tau in the cytoskeleton/insoluble fraction from AD, but not control brains.