Figure 4.

Diagrammatic representation of the workflow involved in Pap test sample preparation for MS analysis. Following a routine Pap test, the SurePath^TM vials were sent to cytopathology for a diagnosis. Excess residual SurePath^TM fluid from women with normal cytology was sent to the research laboratory. Protein concentration was determined by the BCA protein assay, proteins were precipitated with acetone, and visualized with silver stain by SDS-PAGE. Precipitated proteins were also trypsin digested and processed by FASP, and peptides were run on HPLC followed by MS. Data was analyzed by Sequest database searching and Scaffold analysis.