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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Yeast. 2014 Apr 29;31(7):253–264. doi: 10.1002/yea.3014

Table 2.

Plasmids

Plasmid Purpose Source
pFA6a-FRB-KanMX6 to tag gene of interest with FRB at endogenous locus; nourseothricin resistant EUROSCARF: P30578
pFA6a-FRB-GFP-KanMX6 to tag gene of interest with FRB-GFP at endogenous locus; nourseothricin resistant
one XhoI site between FRB and GFP (pFA6a-FRB-(XhoI)-GFP-kanMX6)		 EUROSCARF: P30580
pFA6a-2xFKBP12-His3MX6 to tag gene of interest with FRB-GFP at endogenous locus; histidine prototroph EUROSCARF: P30583
pLD103 pFA6a-2xFKBP12-hph: to tag gene of interest with 2xFKBP12 at endogenous locus; hygromycin B resistant This study
pLD115 pFA6a-FRB-natMX6: to tag gene of interest with FRB at endogenous locus; nourseothricin resistant This study
pLD116 pFA6a-FRB-(XhoI)-GFP-natMX6: to tag gene of interest with FRB-GFP at endogenous locus; nourseothricin resistant This study
pLD133 Episomal expression vector that allows 2FKBP12 to be fused to C-terminus; gene of interest under full strength nmt1 promoter, LEU2 This study
pLD134 Episomal expression vector of rpl13-2FKBP12, under full strength nmt1 promoter, LEU2 This study
pLD138 leu1-32 integration plasmid that allows 2FKBP12 to be fused to C-terminus; gene of interest under full strength nmt1 promoter This study
pLD141 leu1-32 integration plasmid that expresses rpl13-2FKBP12 under full strength nmt1 promoter This study