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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Eur J Immunol. 2014 Apr 23;44(7):2013–2024. doi: 10.1002/eji.201343680

Figure 1. IL-17 production by healthy PPD+ donors and tuberculosis patients.

Figure 1

CD4+ cells and CD14+ cells from freshly isolated PBMC of 10 PPD+ individuals and 10 tuberculosis patients were isolated by magnetic beads conjugated to anti-CD14 or anti-CD4 obtained from Miltenyi Biotech. CD4+ cells and CD14+ cells were cultured at a 9:1 ratio, with or without γ-irradiated M. tb H37Rv for 96 h. IL-17 levels in culture supernatants were measured by ELISA. The horizontal line shows the median, the boxes show the 25th and 75th percentile values, and the whiskers show the 5th and 95th percentile values. Each time experiments were performed with the blood obtained from one PPD+ individual and one tuberculosis patient. A total 10 independent experiments were performed. Maximum, minimum and median values, p values and number of donors (n) are shown.