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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Eur J Immunol. 2014 Apr 23;44(7):2013–2024. doi: 10.1002/eji.201343680

Figure 5. Effect of STAT3 siRNA on M. tb induced IL-17 production and IL-23R expression by CD4+ cells.

Figure 5

CD4+ cells and CD14+ cells from freshly isolated PBMC of PPD+ individuals were isolated by magnetic beads conjugated to anti-CD14 or anti-CD4 obtained from Miltenyi Biotech. Some CD4+ cells were transfected with STAT3 siRNA or scrambled siRNA. After overnight, CD4+ cells and CD14+ cells were cultured at a 9:1 ratio, with or without γ-irradiated M. tb H37Rv for 96 h. A. IL-17 production in culture supernatants was measured by ELISA. Nine independent experiments were performed. B. The percentages of IL-23R+CD4+ cells were measured by flow cytometry. Five independent experiments were performed. Paired and unpaired t tests were performed. Mean values ± SD, p values and number of donors (n) in each panel are shown.