Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Pineal Res. 2014 Jun 28;57(1):90–102. doi: 10.1111/jpi.12146

Figure 7.

Figure 7

Melatonin, NAS, 6-OHM, AFMK, and 5-MT treated keratinocytes decrease CPD formation after UVB exposure. HEKn keratinocytes were treated with melatonin or its derivative for 24 h before UVB exposure. Cells were exposed to UVB intensities of 25 or 50 mJ/cm2 and immediately treated again with melatonin or its derivatives for 3 h. Cells were further fixed and stained with anti-CPD antibody (green) and propidium iodine (PI) (red) (A). Stained cells were imaged with a fluorescence microscope, fluorescence intensity was analysed using ImageJ software, and data are analysed using Graph Pad Prizm after 25 mJ/cm2 (B) and 50 mJ/cm2 (C). Data are presented as percentile of control and were analyzed using t-test, * p<0.05, ** p<0.01, *** p<0.001