Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Pineal Res. 2014 Jun 28;57(1):90–102. doi: 10.1111/jpi.12146

Figure 9.

Figure 9

UVB irradiation increases p53 levels. HEKn keratinocytes were exposed to UVB intensities of 0, 25, 50, or 75 mJ/cm2. Cells were subsequently fixed and stained with anti-p53 antibody (green) and propidium iodine (PI) (red) (A). UVR-irradiated cells showed positive nuclear signal for p53 (A). Stained cells were imaged with a fluorescence microscope; fluorescence intensity was analysed using ImageJ software. Data are analysed using Graph Pad Prizm and expressed as fold change compared to control (0 mJ/cm2). P53 levels increased proportionally to UVB intensity (B). Data are presented as percentile of control and were analyzed using t-test, * p<0.05, ** p<0.01, *** p<0.001.