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. 2014 Apr 29;23(4):457–465. doi: 10.1111/imb.12094

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© 2014 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of The Royal Entomological Society.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Defensin-2 associates with Rickettsia montanensis in vivo. (A) R. montanensis-infected tick midgut incubated with immune sera. (B) R. montanensis-infected tick midgut incubated with control sera. R. montanensis-infected Dermacentor variabilis midguts 48 h post-infection were probed with rabbit defensin and mouse R. montanensis antisera or rabbit and mouse preimmune serum. Rabbit and mouse antibodies were detected with anti-rabbit Alexa-488 (defensin) or anti-mouse Alexa-594 (rickettsia), respectively. SYTOX Blue was used to visualize the nucleus. DIC, differential interference contrast. Bars represent 5 μm.