Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Glia. 2014 May 7;62(9):1418–1434. doi: 10.1002/glia.22689

Figure 3. A) Detection of HSV-1 infection in NPCs.

Figure 3

NPC cultures were labeled with antibody against HSV-1 (red) 24 h pi with 0.2 MOI (d-f) or 2.0 MOI (g-i) HSV-1. Uninfected (a-c) (UI) NPC cultures served as controls. The merge represents the signal of HSV-1, GFP (green; NPCs), and DAPI (blue; nuclei). B) Immunodetection of HSV-1 receptor in uninfected NPC culture using an antibody against HVEM receptor (a: red). The merge (b) represents the signal of HVEM and GFP. NPCs constitutively express the HSV-1 entry receptor HVEM. C) Viral titers of HSV-1 infected NPC cultures. At the indicated time point pi, aliquots of supernatants from NPC cultures infected with 10−4 HSV-1 MOI, 10−2 HSV-1 MOI, or 10−1 MOI HSV-1 were collected and assayed for viral titer. The viral titers are expressed as log PFU ± SEM (n=3 at each time point). Each panel represents two independent experiments with n=3 for each time point.