Table 1. Study design with scaffold type and biomolecule supplementation combinations for each desired lineage.

Two scaffold types (six experimental groups total) were assayed for each lineage. CG and CGCaP scaffolds are sometimes referred to in the text as “nonmineralized” and “mineralized” respectively. Each lineage (tenogenic, chondrogenic, or osteogenic) included use of the standard scaffold group as well as a structurally-modified scaffold (anisotropic, low-density, or CGCaP, respectively). All variants were cultured in MSC growth media. Additionally, the structurally-modified scaffolds (and the standard scaffold in chondrogenic and osteogenic cases) were then cultured with differentiation media or growth media supplemented with growth factors as listed.

Lineage	Biomolecule treatment	Scaffold type, biomolecule dose
Tenogenic		CG (standard)	CG (anisotropic)
	Growth media	Yes	Yes
	bFGF		5 ng/mL
	IGF-1		100 ng/mL
	GDF-5		100 ng/mL
	GDF-7		100 ng/mL
Chondrogenic		CG (standard)	CG (low density)
	Growth media	Yes	Yes
	Chondrogenic	Yes	Yes
	TGF-β1		5 ng/mL
	TGF-β3		5 ng/mL
Osteogenic		CG (standard)	CGCaP (40 wt% mineral)
	Growth media	Yes	Yes
	Osteogenic	Yes	Yes
	BMP-2		50 ng/mL
	BMP-7		50 ng/mL