Table 1. Induction of Conditioning and Sensitization: Roscovitine.
Outline of one 3-day conditioning block used to expose rats to their respective injections during the drug exposure phase of the roscovitine experiment. This 3-day block was repeated four times to complete the 12- day drug exposure phase. During exposure, saline (1.0 ml/kg) and amphetamine (1.5 mg/kg) were administered IP. Roscovitine (40 nmol/0.5μl/side) was administered bilaterally into the NAcc. NAcc microinjections and obturator movements preceded the IP injections by 30-minutes. Following a 1- week withdrawal period, rats were then tested for conditioning (following a saline injection; 1.0 ml/kg, IP) or for sensitization (following an amphetamine injection; 1.0 mg/kg, IP). Roscovitine was not administered on either test. Veh, vehicle. Ros, roscovitine. nc = n/group for conditioning. ns = n/group for sensitization.
| Group | Day 1 - Open Field | Day 2 - Home Cage | Day 3 - Home Cage | |||
|---|---|---|---|---|---|---|
| NAcc infusion |
Systemic injection |
NAcc infusion |
Systemic injection |
No NAcc infusion |
No Systemic injection |
|
|
Paired-Veh
nc=6, ns=6 |
Vehicle | Amphetamine | Obturator Movements |
Saline | Procedure and drug free | |
|
Paired-Ros
nc=7, ns=6 |
Roscovitine | Amphetamine Obturator |
Movements | Saline | ||
|
Unpaired-Veh
nc=5, ns=7 |
Obturator Movements |
Saline | Vehicle | Amphetamine | ||
|
Unpaired-Ros
nc=6, ns=6 |
Obturator Movements |
Saline | Roscovitine | Amphetamine | ||
|
Control-Veh
nc=7, ns=5 |
Vehicle or Obturator Movements |
Saline | Vehicle or Obturator Movements |
Saline | ||
|
Control-Ros
nc=6, ns=5 |
Roscovitine or Obturator Movements |
Saline | Roscovitine or Obturator Movements |
Saline | ||