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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Neurochem. 2014 Feb 12;130(2):268–279. doi: 10.1111/jnc.12659

Figure 2. In vivo treatment with calpain inhibitor SNJ1945 decreases proliferation of primary lymph node cells, reduces secretion of Th cytokines, and supports Th2 cytokines and transcription factor STAT-6.

Figure 2

After immunization, animals were given SNJ1954 (50mg/kg, oral administration) daily on days 9-25 post-induction. Draining lymph nodes (LN) and spleen from control, EAE, and SNJ1945 treated mice were then analyzed. (A) Proliferation analysis of LN via scintillation counter for 3H-thymidine present in the cells as an indicator of DNA replication in the presence or absence of MBP stimulation. (B) The LN cells were grown with a 10-fold excess of irradiated isogenic spleen cells and were stimulated with 40μg/mL whole guinea pig myelin basic protein (MBP). After 48h, 100 μl of supernatant was removed per well for analysis of cytokines by ELISA. (C) Cells stimulated with 40μg/mL MBP were analyzed by FACS for cytokines IL-4, IL-5 and the transcription factor STAT6. N=3, animals in each group. Cells were pooled and one experiment was run for each. *p≤0.05 compared to control; **p≤0.05 compared to EAE.