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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Neurochem. 2014 Feb 12;130(2):268–279. doi: 10.1111/jnc.12659

Figure 3. SNJ1945 treatment effects on myeloid suppressor cells, Th17 cells, and Tregs.

Figure 3

After immunization with MBP, animals were treated with 50mg/kg orally twice daily starting on 9 days post induction and continuing every day until sacrifice. (A) Primary lymph node cells from Control, EAE Vehicle (untreated), and SNJ1945 treated mice were analyzed via flow cytometry for expression of Tregs cells (CD4 + CD25+), Th17 cells (CD4+CCR6+) and MDSCs (GR1+Integrin-α). For each data point, cells were pooled from 4 animals. (B) Spleen cells from the same animals were analyzed by semi-quantiative RT-PCR for inflammatory cytokine IL-17 and anti-inflammatory FoxP3 gene expression for Control, EAE and SNJ1945 groups. The relative density shown is normalized to control. N=4, *p≤0.05.