Frozen spinal cord sections were taken from Control, EAE and SNJ-1945 treated (SNJ) mice, and stained for (A) Axonal degeneration marker dNFP using single staining, 200x magnification. (B) Myelin loss around axons MBP (red) & NFP (green) were shown by double staining, 400x magnification. (C) Neuronal cell death (arrows), TUNEL (red), NeuN (green), and TUNEL-NeuN double staining with DAPI (overlay); 200x magnification.