Table 1.
Results from GZLM model analysis demonstrate significant effects of temperature and L-arginine supplementation on measures of vector competence and on the expression of nitric oxide synthase (NOS). Dashes indicate factors or interaction terms that were either backward eliminated from full models or were not included in the overall experimental design.
| oocyst prevalence (n = 500) |
oocyst intensity (n = 497) |
mosquitoes with sporozoites (n = 476) |
NOS expression (n = 485) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Factors
|
Wald X2
|
d.f.
|
p-value | Wald X2
|
d.f.
|
p-value
|
Wald X2
|
d.f.
|
p-value | Wald X2
|
d.f. | p-value
|
| intercept | 15.54 | 1 | <0.0001 | 4145.89 | 1 | <0.0001 | 1122.16 | 1 | <0.0001 | 27312.86 | 1 | <0.0001 |
| temperature | 29.40 | 4 | <0.0001 | 324.98 | 4 | <0.0001 | 1509.75 | 4 | <0.0001 | 18.52 | 4 | 0.001 |
| supplementation | 18.05 | 1 | <0.0001 | 10.69 | 1 | 0.001 | 294.39 | 1 | <0.0001 | 12.27 | 1 | <0.0001 |
| sampling time point | – | – | – | – | – | – | – | – | – | 580.67 | 3 | <0.0001 |
| replicate | 0.92 | 1 | 0.338 | 30.02 | 1 | <0.0001 | 2.16 | 1 | 0.141 | 0.84 | 1 | 0.360 |
| centred rpS7 cDNA counts | – | – | – | – | – | – | – | – | – | 12.59 | 1 | <0.0001 |
| temperature × supplementation | 1.71 | 4 | 0.789 | 17.15 | 4 | 0.002 | 403.74 | 4 | <0.0001 | – | – | – |
| supplementation × sampling time point | – | – | – | – | – | – | – | – | – | 35.92 | 3 | <0.0001 |
| temperature × sampling time point | – | – | – | – | – | – | – | – | – | 33.32 | 12 | 0.001 |