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(A) ESI-MS of eGFP-CVIA (12). (B) Deconvoluted mass spectrum of eGFP-CVIA. (C) Deconvoluted mass spectra of eGFP-CVIA that was subjected to enzymatic modification using trans-cyclooctene geranyl diphosphate (2) and PFTase. (D) Deconvoluted mass spectra of trans-cyclooctene labeled eGFP-CVIA that underwent tetrazine ligation with benzylamino-tetrazine (4).
