Cytoplasmic dynein IC-2C isoforms were separately transfected into neurons and the fluorescent dynein was imaged as for Figure 2. black, IC-2C WT; white, IC-2C S84A; and gray, IC-2C S84D. Number of axons: WT 47, S84A 44, S84D 36; number of puncta: WT 2867, S84A 1795, S84D 2490.
A. All Motility. For each of the three IC isoforms, the percentage of moving puncta is graphed. Individual dynein puncta were scored as being motile if they showed any displacement between at least two frames. There were significant differences when the motility of S84A puncta was compared to that of the WT or S84D isoforms, *** P<0.001 Student’s t-test. The difference between dynein puncta containing IC-2C WT and IC-2C S84D was not significant
B. Excursive Motility. For each of the three IC isoforms, the percentage of moving puncta exhibiting excursive movement is graphed. Individual dynein puncta were scored as having excursive motility if they showed displacement of at least 1 pixel for four consecutive frames. There was a significant difference when the excursive motility of dynein puncta containing the S84A was compared to that of the WT isoforms, *** P<0.001 Student’s t-test. The differences between S84D and WT or S84A were not significant.