Table I. Motility of dynein complexes with IC-2C WT and phospho-mutants in axons of catecholaminergic neurons.

Catecholaminergic neurons were transfected with the indicated IC-2C mRFP isoform and imaged the next day as described for Figure 2 and in Methods. Each dynein puncta was defined as being either stationary, exhibiting excursive movement, or exhibiting jiggling movement. Excursive movement is movement for at least 4 frames and at least 1 pixel in each frame. Jiggling movement is any movement that does not last for at least 4 frames, and could be as little as 1 pixel movement between 2 frames. The “All Motility” category graphed in Figure 3 is the sum of excursive and jiggling movement.

	IC-2C WT	IC-2C S84A	IC-2C S84D
Number of videos	47	43	35
Total puncta	2867	1795	2490
Stationary puncta	2123	1044	1869
Jiggling movement	647	650	532
Excursive movement	97	101	98
Total observation time (s)	940	860	700