Table II. Kinetics of the motility of dynein complexes with IC-2C WT and phospho-mutants in axons.
IC-2C Isoform | WT | S84A | S84D |
---|---|---|---|
Retrograde Motility | |||
Mean Interval Velocity, μm/sa | 0.89 | 1.02*** | 1.06*** |
(+/− SEM) | (0.02) | (0.02) | (0.02) |
Number | 683 | 780 | 1030 |
Excursion Distance | |||
Mean Distance traveled, μmb | 1.9 | 2.6* | 3.1*** |
(+/− SEM) | (0.24) | (0.19) | (0.22) |
Number | 55 | 67 | 79 |
Pause Time, sec | 2.43 | 2.52 | 2.48 |
(+/− SEM) | (0.23) | (0.15) | (0.16) |
Number | 61 | 74 | 91 |
Anterograde Motility | |||
Mean Interval Velocity, μm/sc | 0.71 | 0.97*** | 1.03*** |
(+/− SEM) | (0.01) | (0.02) | (0.02) |
Number | 1074 | 1172 | 987 |
Excursion Distance | |||
Mean Distance traveled, μmd | 1.22 | 2.43*** | 2.28*** |
(+/− SEM) | (0.09) | (0.21) | (0.17) |
Number | 61 | 88 | 94 |
Pause Time sec | 2.22 | 2.53 | 2.82 |
(+/− SEM) | (0.19) | (0.16) | (0.29) |
Number | 99 | 87 | 61 |
Dynein puncta containing either mutant IC-2C have a greater interval retrograde velocity than WT, *** p<0.001, Student’s t-test.
Dynein puncta containing either mutant IC-2C travel greater distances per retrograde excursion than WT dynein, *** p<0.001, *p< 0.02, Student’s t-test.
Dynein puncta containing either mutant IC-2C have a greater interval anterograde velocity than WT, *** p<0.001, Student’s T-test.
Dynein puncta containing either mutant IC-2C travel greater distances per retrograde excursion than WT dynein, *** p<0.001, Student’s t-test.