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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Neurosci Res. 2014 May 5;92(9):1143–1154. doi: 10.1002/jnr.23388

Table II. Kinetics of the motility of dynein complexes with IC-2C WT and phospho-mutants in axons.

Neurons were transfected with the indicated IC isoform tagged with mRFP and movies of the movement of the fluorescent dynein intermediate chains were obtained as for Figure 2. The movement kinetics for the puncta containing each IC isoform was analyzed and averaged. Interval velocities for dynein puncta with mRFP-IC-2C or mRFP-IC-1B moving in axons were calculated for every movement between each pair of movie frames and averaged. Excursions are puncta movements greater than one pixel for at least 4 frames. Excursion distance is total distance traveled per movement interval. Pauses are periods of time with no dynein movement that separate two excursions. There were no significant differences in pause time for the WT and mutant dyneins (Student’s t-test).

IC-2C Isoform WT S84A S84D
Retrograde Motility
 Mean Interval Velocity, μm/sa 0.89 1.02*** 1.06***
  (+/− SEM) (0.02) (0.02) (0.02)
  Number 683 780 1030
 Excursion Distance
  Mean Distance traveled, μmb 1.9 2.6* 3.1***
  (+/− SEM) (0.24) (0.19) (0.22)
  Number 55 67 79
 Pause Time, sec 2.43 2.52 2.48
  (+/− SEM) (0.23) (0.15) (0.16)
  Number 61 74 91
Anterograde Motility
 Mean Interval Velocity, μm/sc 0.71 0.97*** 1.03***
  (+/− SEM) (0.01) (0.02) (0.02)
  Number 1074 1172 987
 Excursion Distance
  Mean Distance traveled, μmd 1.22 2.43*** 2.28***
  (+/− SEM) (0.09) (0.21) (0.17)
  Number 61 88 94
 Pause Time sec 2.22 2.53 2.82
  (+/− SEM) (0.19) (0.16) (0.29)
  Number 99 87 61
a

Dynein puncta containing either mutant IC-2C have a greater interval retrograde velocity than WT, *** p<0.001, Student’s t-test.

b

Dynein puncta containing either mutant IC-2C travel greater distances per retrograde excursion than WT dynein, *** p<0.001, *p< 0.02, Student’s t-test.

c

Dynein puncta containing either mutant IC-2C have a greater interval anterograde velocity than WT, *** p<0.001, Student’s T-test.

d

Dynein puncta containing either mutant IC-2C travel greater distances per retrograde excursion than WT dynein, *** p<0.001, Student’s t-test.