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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Mol Microbiol. 2014 Jun 15;93(2):276–290. doi: 10.1111/mmi.12658

Figure 1. Purified CdiA-CT536 inhibits E. coli cell growth.

Figure 1

A) Purified CdiA-CT (CT) or CdiA-CT/CdiI (CT/CdiI) complex was added to E. coli X90 cultures at 30 min (indicated by the arrow) and cell growth monitored by measuring the optical density at 600 nm (OD600). B) E. coli X90 cells carrying plasmid-borne cdiI536 or cdiI3937 immunity genes were treated with purified CdiA-CT536 where indicated the arrow. C) Northern blot analysis of CdiA-CT536 treated cells. Total RNA was isolated from the cells in panel B and tRNAICGArg analyzed by northern blot. The migration positions of full-length and cleaved tRNAICGArg are indicated.