Figure 3. CdiA-CT⁵³⁶ toxicity.

Arabinose-inducible cdiA-CT⁵³⁶ expression plasmids were introduced into E. coli X90 and transformants selected on LB-agar supplemented with tetracycline and L-arabinose. CdiA-CT⁵³⁶ requires activation by CysK, therefore E. coli ΔcysK cells were used to control for transformation efficiency. Construct nomenclature corresponds to that introduced in Fig. 2A. The His178Ala mutation ablates tRNase activity and plasmid pCH450 is the empty vector.