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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Mol Microbiol. 2014 Jun 15;93(2):276–290. doi: 10.1111/mmi.12658

Figure 4. CdiA-CTECL from Enterobacter cloacae inhibits E. coli cell growth.

Figure 4

A) Alignment of the CdiA-CT regions from UPEC 536 (Uniprot: Q0T963), Enterobacter cloacae ATCC 13047 (D5CBA0), Yersinia pestis 91001 (Q74T84) and Photorhabdus luminescens TT01 (Q7MB60). Proteins were aligned using Clustal-W and rendered with Jalview 2.8 (Waterhouse et al., 2009) at 30% sequence identity. The conserved cysteine residues within the N-terminal domain are depicted in red. B) Purified CdiA-CTECL was added to E. coli X90 cultures when indicated (downward arrow) and cell growth monitored by OD600 measurements. Where indicated, cells carried plasmid-borne cdiI536 (cdiI536) or cdiIECL immunity genes.