Table 1.
Bacteriophage plating efficiencya
Bacteriophage | ||
---|---|---|
Bacterial strain | R17 | M13 |
X90 ΔtolA | 0.93 ± 0.12 | 0.0b |
X90 ΔtonB | 1.2 ± 0.26 | 1.0 ± 0.20 |
X90 ΔtolA ΔtonB | 1.1 ± 0.15 | 0.0b |
X90 ΔtraA::cat pTrc | 0.0b | 0.0b |
X90 ΔtraA::cat pTrc::traA | 1.2 ± 0.18 | 1.2 ± 0.15 |
X90 ΔtraA::cat pTrc::traA(D74G) | 0.0b | 1.1 ± 0.09 |
X90 ΔtraA::cat pTrc::traA(G120C) | 0.09 ± 0.005c | 0.77 ± 0.06c |
X90 ΔtrbI::cat | 0.1 ± 0.04c | 0.1 ± 0.03c |
The indicated bacterial strains were infected with 10 and 100 plaque forming units (pfu). The number of plaques for each strain was divided by the plaques obtained with wild-type X90 cells. The mean plating efficiency ± SEM is reported (n = 4).
No plaques were detected when bacteria were incubated with 103 pfu of phage.
Plaques were turbid.