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. 2014 Jul 5;5(7):572–584. doi: 10.7150/jca.8865

Figure 1.

Figure 1

tEV treatment of differentiating miPSCs gives rise to stem-like cells. (A) Cells are passaged following the conversion schedule. Each color indicates different culture media. (B) Size distribution of tEVs collected from LLC CM. (C) Immunoblotting analysis of CD63 in tEVs and LLC cell lysates shows the enrichment of exosomes. Coomassie stain of SDS-PAGE gel shows equal loading of total protein. (D) Colony formation in indicated concentrations of tEVs cultured for 2 weeks. (E) Cell images during conversion by 100ng/mL tEV are shown. Cells passaged in plain medium (-tEV) are used as control. Scale bar: 100 µm. (F) Immunoblotting analysis of Nanog and Oct3/4 in the total protein from miPSCs (miPS), differentiated miPSCs (differentiated miPS (9days)), differentiated cells by tEVs cultured for 6 days (miPS + tEV (6days)), differentiated cells by tEVs cultured for 28 days (miPS + tEV (28days)) and resultant cells (miPS-LLCev). Relative intensities are normalized to that of β -actin.