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. 2014 Jul 5;5(7):572–584. doi: 10.7150/jca.8865

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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miPS-LLCev generate liposarcoma-like tumors in immunodeficient mice. (A) Generation of tumors after subcutaneous injection shows rapid growth of tumors. (B) H&E staining of primary tumors dissected from injection site, showing multiple pathologic mitotic figures (arrowheads, inset) and hyper-vascularization. (C) IHC staining of Ki67 shows cellular proliferation. (D) IHC staining of CD31 shows angiogenesis in tumors (arrowheads). Serial sections were stained with vimentin (E) and GFP (F), diffuse staining of vimentin is detected with opposite pattern to GFP in tumor. (G) IHC staining of PPARγ2 shows a large population of PPARγ2 expressing cells. (H) Oil Red O staining shows fat droplets in tumors. Scale bars: 100 μm.