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. 2014 Jul 5;5(7):572–584. doi: 10.7150/jca.8865

Figure 6.

Figure 6

Cells derived from tumors possess heterogeneous populations. CD34 (A), CD36 (B), PPARγ2 (C) immunofluorescence staining of primary cells show different stages of adipocyte differentiation in primary bulk cell culture. Arrowheads are showing the double positive cells. (D) In virto tube formation assay shows both PT cell and DT cells possess tube formation ability. The CD31 positive and negative cells indicate the heterogeneity of primary cells. (E) Oil Red O staining after in vitro differentiation of spheroid cells, shows the two primary cell lines could differentiate into adipocytes. (F) Matrigel invasion assay shows significantly higher invasive capacity of both primary cells than resultant cell. DT cell line is slightly more invasive than the PT cell line. A, B and C scale bars: 10 μm. D and E scale bars: 100 μm. F scale bar: 200 μm.