Skip to main content
. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 13;193(2):519–528. doi: 10.4049/jimmunol.1301668

Figure 3. Biosensor signal correlates with gold standard 51Cr release assay.

Figure 3

In vitro CTLs were generated by antigen stimulation of splenocytes from 4 individual P14 TCR transgenic mice. CTLs were either co-incubated with biosensor expressing, cloned EL4 cells or 51Cr loaded EL4 target cells at effector/target ratios ranging from 12:1 to 0.19:1. 51Cr release was measured after 4 hours. (A) RLU at 30 minutes of the luminescence assay for all 4 mice with titration of E/T ratio; (B) 51Cr release percentage for all 4 mice with titration of E/T ratio; Best fit curves were logarithmic for A and B. (C) Correlation between biosensor assay and 51Cr assay. Data is representative of 3 independent experiments.