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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: Biochem Pharmacol. 2012 Apr 10;84(2):232–239. doi: 10.1016/j.bcp.2012.04.002

Fig. 4. Hydrolysis of aspirin, clopidogrel and 1-naphthylacetate by recombinant carboxylesterases.

Fig. 4

Cells (293T) were transfected with a carboxylesterase expression construct or the corresponding vector. The transfected cells were cultured for 24 h and cell lysates were prepared by sonication. Hydrolytic activity of cell lysates (20 μg) was determined toward aspirin (A), clopidogrel (B) or 1-naphthylacetate (C). The hydrolysis of aspirin and clopidogrel was monitored by HPLC whereas the hydrolysis of 1-naphthylacetate by spectrophotometry from an increase in absorbance at 322 nm with 1 mM 1-naphthylacetate. The results were collected from three separate experiments. The data are expressed as mean ± SE (standard error).