Fig. 5. Lipid staining by Oil Red O.
Cells (293T) were transfected with a carboxylesterase expression construct or the corresponding vector. The transfected cells were cultured for 24 h and then cultured in fatty acid-rich medium. Cells were fixed and stained with Oil Red O as described in the section of Materials and Methods by microscopic or spectrophotometric analyses. The data are expressed as mean ± SE (standard error).