Figure 5.

NAG neurons exhibit an adult-like phenotype in the presence of leptin after postnatal day 30. Representative traces from brain slices from NPY-hrGFP mice containing ARH. A, Leptin 100 nm leads to depolarization in 5 of 19 neurons and hyperpolarization in 6 of 19 neurons from 12 animals at P25–P27. B, Leptin causes membrane hyperpolarization and inhibition of spontaneous action potentials in 5 of 8 neurons from 4 animals at P30. C, Leptin 100 nm causes membrane hyperpolarization in 12 of 21 adult neurons from 10 animals. Bar graphs represent the magnitude of leptin responses in neurons for each age. Line graphs represent changes in membrane potential for individual neurons that were sensitive to leptin 100 nm at each age tested. Results are mean ± SEM. **p < 0.01 (paired t test). ***p < 0.001 (paired t test). ****p < 0.0001 (paired t test).