Fig. 5.
Histomophometric determination of trabecular bone volume and TRAP-positive cell number. Longitudinal distal femoral sections from Ext1f/f and Ext1f/f;Col2CreERT mice tamoxifen-injected at P5 and harvested at 2 weeks were stained with H&E or TRAP (4 controls and 4 mutants) and subjected to histomorphometry. (A) Trabecular bone volume in primary spongiosa was measured and normalized over total volume using BioQuant OSTEO 2011 Protocol. (B) TRAP staining positive cells along the chondro-osseous junction were counted and normalized to overall length, using Image J. Note that both parameters changes in mutants versus controls in a statistically significant manner (*p<0.05).