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. 2013 Jan 15;8(2):111–120. doi: 10.3969/j.issn.1673-5374.2013.02.002

Figure 8.

Figure 8

Effects of calmodulin-dependent protein kinase II inhibitor KN-93 on phospho-CaMKII (P-CaMKII) and total-CaMKII levels in cultured cortical neurons induced by 50 μM NMDA.

I–VI: Sham, NMDA, 0.5 μM KN-93, NMDA + 0.25 μM KN-93, NMDA + 0.5 μM KN-93, NMDA + 1.0 μM KN-93 groups.

(A) Representative western blot of P-CaMKII (54 kDa) and total-CaMKII (50 kDa) in cultured cortical neurons exposed to NMDA.

(B) Quantification of P-CaMKII and total-CaMKII expression. P-CaMKII and total-CaMKII were determined by calculating the ratio of absorbance value relative to β-actin.

Data are expressed as mean ± SD, n = 6 for each group. aP < 0.01, vs. sham group; bP < 0.01, vs. NMDA group using one-way analysis of variance.

NMDA: N-methyl-D-aspartic acid; CaMKII: calmodulin-dependent protein kinase II.