Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 5;8(13):1157–1168. doi: 10.3969/j.issn.1673-5374.2013.13.001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Double fluorescence staining for the P2X4 receptor, P2X7 receptor, interleukin (IL)-1α and IL-1β expression in astrocytes and microglia in hippocampal slices subjected to oxygen-glucose deprivation (confocal laser scanning microscope, × 100).

At 75 minutes after oxygen-glucose deprivation, in green astrocytes (Cy2-stained; glial fibrillary acidic protein, GFAP), P2X4 and P2X7 receptors were stained red with Cy3. After merging the two images, yellow staining appeared (arrows; A) indicating colocalization. IL-1α and IL-1β were not stained with Cy3. After merging of these images, no colocalization was observed (arrows; B). In red microglia (Cy3 stained; OX42), P2X4 receptor, P2X7 receptor, IL-1α and IL-1β all stained green with Cy2, and after merging of the images yellow double staining appeared (arrows; C, D), indicating colocalization.